Isolation and Characterization of a New Thermoalkalophilic Lipase from Soil Bacteria

نویسندگان

چکیده مقاله:

Lipases are diversified enzymes in their properties and substrate specificity, which make them attractive tools for various industrial applications. In this study, an alkaline thermostable lipase producing bacteria were isolated from soil of different regions of Isfahan province (Iran) and its lipase was purified by ammonium sulfate precipitation and ion exchange chromatography. To select a thermoalkalophil lipase producing bacterium, Rhodamine B and Horikoshi media were used and the strain that can grow at 45 °C was selected. The isolated strain was identified using microbial and biochemical tests. One strain showed an orange colored zone on plate and grew on Horikoshi plate. Microbial and biochemical tests showed that the isolated strain was Bacillus subtilis, a Gram positive rod. In PCR, an expected band was obtained with about 371 bp. The activity of the purified lipase was 10.2 folds that of the standard enzyme using ammonium sulfate precipitation and ion exchange chromatography. The molecular weight of lipase determined by SDS-PAGE electrophoresis, was 21 and 35 KDa. Existence of two bands in SDS-PAGE electrophoresis and low amount of obtained purified enzyme highlights the necessity of optimization of purification and concentrating process.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

isolation and characterization of a new thermoalkalophilic lipase from soil bacteria

lipases are diversified enzymes in their properties and substrate specificity, which make them attractive tools for various industrial applications. in this study, an alkaline thermostable lipase producing bacteria were isolated from soil of different regions of isfahan province (iran) and its lipase was purified by ammonium sulfate precipitation and ion exchange chromatography. to select a the...

متن کامل

Isolation, Optimization, and Molecular Characterization of a Lipase Producing Bacterium from Oil Contaminated Soils

Lipases have many applications in biotechnology, thanks to their ability of acylglycerides hydrolysis. They alsp possess the unique feature of acting at the lipid-water interface, which distinguishes them from esterases. Commercially useful lipases are produced by microorganisms with the extracellular lipase being produced by many bacteria including Pseudomonas. The greatest production of lipas...

متن کامل

Isolation and characterization of phenol degrading bacteria from Persian Gulf

Phenol and phenol compounds are environmental pollutants present in industrial wastewaters such as, coal tar, oil refineries and petrochemical plants exist. Phenol removal from industrial effluent is extremely important in protection of environment. Recently phenol biodegradation has been considered. Marine bacteria are the most important phenol biodegrader. In this study, the phenol-degrading ...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 14  شماره 3

صفحات  901- 906

تاریخ انتشار 2015-06-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023